Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Molecular Devices SpectraMax M5e Microplate Reader. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). MMP‑1 inhibition assay. Heme was preincubated for 5 min with A when added before the metals. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. 2. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Dissolve 13. 190–850 nm <± 1. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. 2 software was used to measure and calculate of the bioactivity activity studies. Amino Acid Analysis. AoSMCs were seeded into 6-well culture plates. To correct for inter. used in the SPECTRAmax PLUS. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). TDP-43 stress granular cells were treated with 70% ice-cold. 0. Donor: 620 nm. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. View the Molecular Devices website. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Data were expressed as the mean LPS-treated cells (100%) vs. Awareness Technology Inc. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Each experiment was performed in duplicate. 305 reference level saturation. The plate was covered and incubated for one hour at room temperature. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Call 1-800-745-2710. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Stat Fax 3200 Microplate Reader. 5. SpectraMax Microplate Readers. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Data are expressed as percent of maximum binding for each protein. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. 024). Read one sample or up to 384. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). 6 g sodium acetate trihydrate and 2. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Fully tested with Warranty. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). TDP-43 stress granular cells were treated with 70% ice-cold. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). MOLECULAR DEVICE M5. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. MCE has not independently confirmed the accuracy of these methods. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. 2. TGFα release was calculated as described in the Results. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. StakMax. , Sunnyvale, CA, USA) at 595 nm. Acceptor filter: 665 nm. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. G. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. These lab workhorses are used in drug discovery, research, bioassay validation, and. 2. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 5. , Sunnyvale, CA) at 490 nm. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. 2. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Operators can conveniently select up to 6 wavelengths at a time for. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. Quick View. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Clotting times (mean ± SD) for rSimukunin and rSV. Homeostatic model assessment for insulin. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Figure 1. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 6. 5% phosphoric acid) in microplates at room temperature for 10 min. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. chen@case. . P. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Rate of fibrinolysis. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. 00 USD. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Data were expressed as the mean percent viable cell vs. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . Molecular Devices. Emission. ATI/240, 250. Stat Fax 3200 Microplate Reader. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. The light features a slim profile to prevent. 2 Chemical Reagents 1. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Samples were analyzed in triplicates. Molecular Device M2e. 30. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). 0 1. The. The plate was covered and incubated for one hour at room temperature. 2 M DEEMM were mixed in a microtube,. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Endpoint, kinetic, spectrum. Each experiment was performed in duplicate and repeated at. Molecular Devices Spectramax 340PC instructions. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. control. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). 5. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. 190-1000 nm, tunable in 1 nm increments. The. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Manager: Yinghua Chen, PhD Contact Email: yinghua. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. (1988) and recorded at 412 nm. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Details. 0. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Quick View. SpectraMax 384 Plus. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Plates were washed with 1× KP solution and blocked with. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Fibrin clots were formed as described above with some modifications 46. Molecular Devices SpectraMax M5e Microplate Reader. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. control or celatrol-treated cells. 5 mL sample or standard solution was well mixed with 0. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. using SpectraMax i3 (Molecular devices, San Jose, USA). Molecular Devices SpectraMax Gemini XS Microplate Reader. 1% N-1-naphtylethylenediamine dihydrochloride, 2. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0% and ± 0. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. plates can be used in the SPECTRAmax PLUS 384. 0% and ± 0. #ABS PLUS) Methods. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. As a common indicator of equine T cell activation, cytokine induction. 1 mL of acetylacetone. 1 0 100 80 60 40 20 0 4. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 97), and each plate contained at least six positive and six negative controls. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The reaction mixture was incubated for 5 min. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. edu Contact Phone: 216-368-8962. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Examples: “Press carefully to engage,” “Do not press down. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. A. Subsequent replacements require only the lamp (N-34-LB) be replaced. , Sunnyvale, CA). The first time you replace an OEM lamp you must use the N-34-MLA. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. Details. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. SoftMax Pro. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Statistical analysis. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. Details. Awareness Technology, Inc. Other technologies such as imaging. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Nitric Oxide Assay. 20. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. A. . Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. 304 signal level saturation. Each experiment was performed in duplicate and repeated at. Donor: 616/10 nm. Filterless readers provide consistent results with steady temperature regulation. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. SpectraMax Multi-Mode Microplate Readers. Microplate Readers. 340 nm / 70 nm. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. SPECTRAmax® 190. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. reader (Molecular Devi ces). Molecular Device M5e. Near bottom. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Molecular Devices Corporation 1311. , Sunnyvale, CA, USA) at a wavelength of 450 nm. 1 mL of acetylacetone. Wavelength Selection. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. reader (SpectraMax 340; Molecular devices, LLc). Samples were plated in duplicate (r=0. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. In vivo anti-malarial assay in mice. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. 85%. Unit powers. Values were determined according to a standard curve (log/log curve-fit). Softmax PRO v5. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. Or scan up to 384 wells at any time to determine the best assay conditions. , Sunnyvale CA). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 2 were considered negative for IgM antibody. S. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). Molecular Devices SpectraMax Gemini XS Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Samples containing 25 μg of protein were separated on 12. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 2. Amino Acid Analysis. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 003 OD Stray light ≤ 0. Awareness Technology Inc. 4. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The reaction mixture was incubated for 5 min. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. edu Contact Phone: 216-368-8962. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. N-34-LB. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Not. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 05 ng/mL; progesterone: 2. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. SpectraMax Multi-Mode Microplate Readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Also for: Spectramax 190, Spectramax 340pc384, Versamax. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). A 0. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Percent viability was calculated by comparison with the control group. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax Multi-Mode Microplate Readers. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 2. #N207) LAL Reagent Water (Lonza cat. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 6. 0 3. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Driessen*,MaaikeA. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. 06 ng/mL; FSH: 0. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Molecular Devices Spectramax 340 Plate Reader Repair. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. 2 0. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. ScanLater Western Blot Detection Cartridge. All strains were tested three times and the results averaged. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 9000-0756. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. 7. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Labsystems Luminoskan RT Microplate Reader. Upgradeable modules are also available including western blot, cell imaging, and fast. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 8. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Product Keyword. Service Contracts, Preventative Maintenance and Performance. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Cytotoxicity was determined by measuring the release of LDH. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. #N207) LAL Reagent Water (Lonza cat. The VersaMax and SpectraMax 190 read 96-well microplates. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. (7. VersaMax reads 96-well and strip-well plates. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 0, 20 mmol L –1cal-cium chloride). 94 g calcium chloride dihydrate (both. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. 303 unable to cal dark current. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 3 mm. Six wells were assayed for each set of conditions and stadard deviations. No power cord or accessories included. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. 6. 10. 2. The metals were preincubated with A for 5 min when added before heme.